Product name: Human shRNA Clone
Specifications: 500ul Glycerol Stock plus 5ug DNA
Shipping & Storage Conditions：
Product shipped at ambient temperature. Upon receiving, please store at -20 degree(for Plasmid) -80 degree(for Glycerol Stock) for long-term storage.
Product features：
1.For each shRNA, tranfection has been tested, green fluorescent has been observed;
2.All the shRNA clones includes ampicillin gene for positive clone selection. Its recommended working concentration is 50 ug/mL.
Handling instruction：
For glycerol stock, streak the bacteria onto an LB agar plate. Incubate at 37 °C for overnight. Inoculate a single colony into fresh LB medium and culture overnight at 37 °C in a shaking incubator. Then extract the plasmid and sequence verification.
For plasmid DNA, the Plasmid DNA product is usually provided as as liquid. Therefore, the Plasmid DNA is easily adsorbed on the surface of the tube during shipping. We strongly recommend spining the tube briefly to ensure that the liquid is at the bottom of the tube before opening. The Plasmid DNA product isn’t directly recommended for cell transfection. You can perform the transformation of the plasmid DNA and use a transfection-grade plasmid extraction kit for high-quality plasmid DNA preparation. Or you can choose our company's transfection-grade plasmid preparation service.
Note：If you have any questions about how to use the product, please contact our technical support. Our email address is techsupport@wzbioscience.com.
ATGTCTGGAATCAAGCGAACCATCAAAGAAACCGACCCTGATTACGAGGATGTATCTGTGGCCCTTCCAAATAAGCGGCATAAAGCAATTGAGAATTCAGCTCGAGATGCTGCTGTGCAGAAGATTGAGACTATTATCAAAGAACAGTTTGCTCTTGAAATGAAGAATAAGGAACATGAAATTGAAGTCATTGACCAGCGACTGATTGAAGCAAGAAGGATGATGGATAAACTGCGTGCCTGCATTGTAGCAAACTACTATGCTTCTGCAGGTCTTCTAAAAGTTTCTGAGGGATCAAAGACATGTGATACAATGGTTTTTAATCATCCTGCTATCAAGAAATTTTTGGAATCACCATCTAGGTCATCATCTCCTGCCAATCAGAGAGCAGAAACACCATCAGCCAATCATTCAGAAAGTGATTCTTTATCTCAGCACAATGACTTCTTATCTGACAAAGATAATAACAGCAATATGGATATAGAGGAAAGACTCTCAAACAACATGGAGCAGAGACCAAGCCGAAATACTGGAAGGGATACTTCTAGAATTACTGGCTCCCATAAAACAGAACAGCGGAATGCTGATCTCACAGATGAGACTTCACGACTTTTTGTAAAGAAAACAATAGTAGTGGGCAATGTGTCCAAGTATATACCTCCGGATAAGAGGGAAGAAAATGACCAGTCAACTCATAAGTGGATGGTATATGTCCGAGGGTCCCGTAGAGAACCCAGCATTAATCATTTTGTCAAGAAGGTTTGGTTCTTCCTTCATCCTAGCTATAAACCAAATGACCTTGTGGAAGTTAGAGAGCCTCCTTTTCACCTGACCAGAAGAGGCTGGGGTGAGTTTCCCGTCAGAGTTCAAGTTCATTTTAAGGACAGCCAGAACAAGCGGATAGATATCATACATAATCTGAAGCTGGATAGAACTTATACTGGCTTGCAGACTCTTGGAGCAGAGACGGTAGTGGATGTTGAACTCCATCGCCATTCTCTCGGAGAAGACTGTATCTATCCTCAGTCCTCGGAGTCTGACATCTCTGATGCCCCTCCATCTTTGCCTTTGACCATTCCAGCCCCAGTGAAAGCTTCTTCACCAATAAAGCAGTCACATGAGCCAGTACCCGATACCTCTGTGGAGAAAGGATTCCCAGCTAGCACTGAAGCTGAACGACACACTCCGTTTTATGCTTTGCCATCTTCATTGGAAAGAACACCCACCAAAATGACTACATCCCAGAAAGTTACCTTTTGTTCCCATGGCAATTCAGCTTTCCAGCCAATAGCATCAAGCTGCAAAATTGTTCCACAAAGTCAGGTTCCTAATCCTGAGTCACCTGGAAAATCCTTCCAGCCCATCACCATGAGCTGCAAGATTGTGTCAGGTTCCCCAATATCAACTCCAAGCCCATCACCATTGCCTCGAACCCCGACTTCCACTCCAGTCCACGTGAAGCAAGGCACTGCCGGCTCTGTTATTAATAATCCTTATGTTATCATGGACAAGCAGCCGGGGCAGGTGATTGGAGCCACCACTCCCAGTACAGGAAGTCCTACAAACAAGATCTCCACGGCTTCTCAGGTCTCCCAAGGAACAGGTTCCCCTGTTCCTAAAATTCATGGAAGTAGTTTTGTAACATCTACTGTCAAGCAGGAGGATTCTTTGTTTGCATCTATGCCACCTCTTTGCCCAATTGGGAGTCACCCTAAGGTTCAAAGCCCCAAACCTATAACAGGAGGACTTGGAGCTTTCACAAAAGTGATCATCAAACAGGAACCTGGTGAAGCCCCTCACGTGCCCGCAACAGGAGCTGCCAGCCAGTCACCACTCCCGCAGTATGTGACTGTGAAAGGGGGTCACATGATAGCTGTGTCCCCTCAAAAACAGGTCATAACTCCTGGAGAAGGGATTGCCCAGTCAGCAAAGGTTCAGCCCTCCAAGGTTGTCGGGGTACCAGTTGGGTCTGCTTTACCTTCAACAGTGAAGCAGGCTGTGGCGATCAGTGGTGGCCAGATCCTGGTAGCCAAGGCCAGCTCTTCTGTCTCCAAAGCAGTTGGGCCAAAGCAAGTTGTAACCCAAGGAGTTGCCAAAGCAATTGTGAGTGGAGGTGGAGGAACCATTGTTGCTCAGCCAGTGCAGACCTTAACCAAGGCCCAGGTTACTGCCGCTGGTCCTCAGAAGAGTGGATCCCAGGGTTCAGTAATGGCAACGTTGCAGCTACCAGCCACTAATTTGGCCAACTTGGCAAATTTGCCTCCTGGCACTAAACTCTACCTAACTACAAACAGCAAGAACCCTTCAGGAAAAGGAAAACTGCTGCTGATCCCTCAAGGAGCCATCCTGCGAGCTACGAACAATGCTAATCTCCAGTCTGGCTCAGCTGCCAGTGGTGGGAGTGGTGCCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCGGCAGTGGCAGCGGTGGAGGCGGCAGCACAGGAGGAGGAGGAGGAACAGCAGGAGGAGGAACTCAAAGTACTGCTGGCCCTGGAGGGATATCTCAGCACCTGACTTACACATCTTACATCCTCAAGCAAACTCCCCAGGGCACATTTTTAGTTGGCCAGCCATCACCCCAGACTTCTGGAAAACAACTCACCACTGGGTCAGTGGTCCAAGGAACACTGGGAGTCAGCACATCTTCTGCACAAGGACAACAAACGCTAAAAGTCATCTCTGGACAGAAAACCACATTGTTTACACAGGCAGCCCATGGAGGACAGGCATCTCTAATGAAAATATCCGATAGCACCTTGAAGACTGTGCCAGCCACCTCACAGCTCTCGAAGCCTGGAACCACAATGCTGAGAGTAGCAGGAGGGGTTATCACAACTGCCACTTCCCCTGCCGTGGCCCTCTCAGCAAACGGTCCTGCACAACAGTCTGAAGGAATGGCTCCCGTGTCTTCATCTACGGTCAGTTCTGTAACGAAAACTTCTGGGCAGCAGCAAGTGTGTGTGAGCCAGGCCACCGTGGGAACCTGCAAGGCTGCCACCCCCACCGTCGTCAGCGCCACGTCCCTCGTGCCTACACCAAACCCCATCTCTGGGAAAGCCACAGTATCCGGACTGTTAAAGATTCACTCCAGTCAGTCCAGTCCGCAGCAGGCCGTCCTGACGATTCCCAGCCAGCTCAAACCACTCAGCGTAAACACATCTGGAGGGGTGCAGACGATCCTGATGCCTGTGAATAAAGTGGTTCAGTCATTTTCTACCAGCAAGCCACCTGCCATTCTGCCTGTAGCTGCCCCAACTCCAGTTGTCCCCAGCTCTGCTCCAGCAGCTGTTGCAAAAGTGAAGACTGAACCAGAAACACCTGGACCGAGTTGCCTCTCTCAGGAGGGTCAGACAGCAGTGAAAACAGAAGAAAGTTCTGAGCTGGGAAACTATGTCATTAAGATAGACCATTTAGAAACTATCCAGCAACTCCTAACTGCAGTAGTAAAGAAGATTCCATTAATCACTGCAAAAAGTGAAGATGCCAGCTGCTTTTCTGCAAAGTCTGTGGAGCAGTACTATGGCTGGAACATTGGAAAAAGGAGAGCCGCTGAGTGGCAAAGAGCAATGACAATGCGAAAAGTCTTACAAGAAATCCTGGAGAAGAATCCGAGATTTCACCACCTGACTCCCCTCAAAACCAAGCACATCGCTCACTGGTGCCGCTGTCATGGCTACACCCCACCGGACCCTGAGAGCCTGAGGAATGACGGGGACTCCATCGAGGACGTGCTGACCCAGATCGACAGCGAGCCCGAGTGCCCATCATCATTCTCCTCTGCTGACAACCTCTGCCGCAAACTGGAGGACCTGCAACAGTTCCAGAAAAGGGAACCCGAGAATGAGGAGGAGGTGGACATCCTCAGCCTCTCCGAGCCAGTGAAGATAAACATCAAGAAGGAGCAGGAAGAGAAACAAGAGGAAGTCAAGTTCTACCTGCCACCAACCCCAGGGTCTGAATTTATTGGGGATGTCACACAGAAGATTGGGATCACCCTGCAGCCCGTGGCACTCCACAGGAACGTGTATGCGTCCGTGGTGGAGGACATGATCCTGAAGGCTACAGAACAGCTGGTGAATGATATCCTGAGACAGGCTTTGGCAGTTGGATACCAGACAGCTTCTCACAACAGGATTCCCAAAGAAATTACAGTGAGTAATATTCACCAGGCCATTTGCAACATTCCTTTTCTGGACTTCCTCACAAACAAACACATGGGAATATTGAATGAGGACCAG
siRNA1： AAAGCAATTGAGAATTCAGCTCG
siRNA2： AATCTGAAGCTGGATAGAACTTA
siRNA3： AATTGGGAGTCACCCTAAGGTTC
siRNA4： AAACAACTCACCACTGGGTCAGT
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